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Revista Portuguesa de Estomatologia,
Medicina Dentária e Cirurgia Maxilofacial

rev port estomatol med dent cir maxilofac. 2019;60(4):163-168

Original research

Evaluation of the buccal mucosa of patients with
acute lymphocytic leukemia: A case series study

a,b
c
a,b
Juliana Maria Souza de Oliveira , Juliana Vianna Pereira , Évelyn Costa Lira ,
b
c
Cristina Maria Borborema dos Santos , Gerson de Oliveira Paiva Neto ,
Tatiana Nayara Libório-Kimura b,d, *
a Faculdade de Odontologia. Universidade Federal do Amazonas, Manaus, AM, Brasil
b Programa de Pós-Graduação em Odontologia (PPGO). Universidade Federal do Amazonas, Manaus, AM, Brasil
c Centro de Apoio Multidisciplinar (CAM). Universidade Federal do Amazonas, Manaus, AM, Brasil
d Departamento de Patologia e Medicina Legal. Faculdade de Medicina. Universidade Federal do Amazonas, Manaus, AM, Brasil

a r t i c l e i n f o a b s t r a c t

Article history: Objectives: Prospective evaluation of the buccal mucosa of pediatric patients with acute lym-
Received 18 September 2019 phocytic leukemia (ALL) regarding its clinical aspect and expression of members from the HHV
Accepted 6 December 2019 family.
Available online 3 January 2020 Methods: From September 2014 to January 2015, a series of nine consecutive patients was
evaluated, with ages ranging from 2 to 14 years, in treatment at the Amazon Hematology and
Keywords: Hemotherapy Foundation (HEMOAM). The buccal mucosa of those patients was clinically
Acute lymphocytic leukemia evaluated, screened for alterations and analyzed by PCR, to search DNA from HSV-1, CMV and
Herpesviridae EBV during four moments of the pre-phase/induction phase of the chemotherapy treatment
Oral mucositis (P/I) – D0/D1, D8, D15 and D35, as well as four moments of the consolidation of the remission
Polymerase Chain Reaction phase (CR) – D1, D15, D29 and D50. The protocol proposed by the Brazilian Cooperative Group
for Treatment of Childhood Lymphocytic Leukemia (GBTLI ALL-2009) was followed.
Results: A prevalence of 2-year-old children (66.7%, n=6) with a diagnose of B-cell ALL (88.9%,
n=8) was observed. Buccal alterations were observed in 33.3% (n=3) of the patients: erythema
(D35 P/I), dry lips (D8 and D15 P/I) and an episode of xerostomia (D15 P/I). None of the samples
was positive for HSV-1 and CMV, but 33.3% (n=3) of the cases expressed EBV (D8 and D15 P/I).
Conclusions: Buccal alterations and the presence of HSV-1, CMV and EBV in patients with
ALL was inexpressive, with most of the patients being sound throughout the treatment.
Thus, it cannot be affirmed that the analyzed viruses are part of the microbiome of those
patients. However, it has been suggested that the presence of EBV is more expected than
HSV-1 and CMV. (Rev Port Estomatol Med Dent Cir Maxilofac. 2019;60(4):163-168)
© 2019 Sociedade Portuguesa de Estomatologia e Medicina Dentária.
Published by SPEMD. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).

* Corresponding author.
E-mail address: tliborio@ufam.edu.br, tatiana.liborio@gmail.com (Tatiana Nayara Libório Kimura).
http://doi.org/10.24873/j.rpemd.2020.01.691
1646-2890/© 2019 Sociedade Portuguesa de Estomatologia e Medicina Dentária. Published by SPEMD.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).