Page 23 - SPEMD_60-3

Revista Portuguesa de Estomatologia,
Medicina Dentária e Cirurgia Maxilofacial

rev port estomatol med dent cir maxilofac. 2019;60(3):111-117

Original research

Cytotoxic effects of hydrogen peroxide
on periodontal cells

a,b
a,b
a,b
Andreia Vieira , Joana Marques a,b, *, Mariana Cruz , Carlota Mendonça ,
Duarte Marques a,b,c , António Mata a,b,c
a Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal
b GIBBO – Grupo de Investigação em Biologia e Bioquímica Oral – Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal
c LIBPhys-FCT UID/FIS/04559/2013

a r t i c l e i n f o a b s t r a c t
Article history: Objectives: To evaluate the in vitro cytotoxic effects of hydrogen peroxide (H O ) on perio-
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Received 20 April 2019 dontal cells, based on the cellular viability and morphology of immortalized osteoblast and
Accepted 10 September 2019 gingival fibroblast cultures, with different exposure times and concentrations.
Available online 30 September 2019 Methods: Immortalized human gingival fibroblast and human fetal osteoblast cell lines were
cultured, separately, in 96-well plates. After reaching confluency, they were exposed to H O
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Keywords: solutions at 16 different concentrations ranging between 0.05 µg/ml and 10 µg/ml for 1 h,
Cytotoxicity 24 h or 72 h in triplicate assays (n=24), using culture media alone as the control. Cell viabil-
Fibroblast ity was measured by previously established fluorometric methods, using a resazurin-based
Gingiva assay, and cell morphology by using an inverted microscope with integrated phase-contrast
Hydrogen peroxide optics. Data were statistically analyzed using a one-way ANOVA with Tukey’s and Dunnet’s
Osteoblast post hoc tests and Pearson correlation coefficient (r), as appropriate (α=0.05).
Results: H O induced a decrease in cell viability to below 50% in fibroblasts and around 50%
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in osteoblasts, in all tested concentrations after 1h exposure, and a decrease in cell viabili-
ty to above 70% after 24 h and 72 h (P<0.05). A significant negative correlation was detected
between H O and cell viability at 1 h and 72 h for osteoblast (r=-0.471) and HGF (r=-0.12)
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2
cells, respectively. The cell morphology analysis showed cell detachment and lower cell
density, in agreement with these findings.
Conclusions: H O induced cell alterations with moderate to severe cytotoxic effects in osteo-
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blast and gingival fibroblasts. (Rev Port Estomatol Med Dent Cir Maxilofac. 2019;60(3):111-117)
© 2019 Sociedade Portuguesa de Estomatologia e Medicina Dentária.
Published by SPEMD. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).

* Corresponding author.
E-mail address: joanafariamarques@gmail.com (Joana Faria Marques).
http://doi.org/10.24873/j.rpemd.2019.09.455
1646-2890/© 2019 Sociedade Portuguesa de Estomatologia e Medicina Dentária. Published by SPEMD.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).